Determination of amoxicillin in plasma by HPLC with fluorescence detection after on-line oxidation
Summary
Type: HPLC Application #: 103560
Detection: fluorescence, excitation 255nm, emission 400nm after postcolumn electrochemical oxidation at 0.78V Conditions: eluent: 0.02M methanesulphonic acid - acetonitrile (92.5:7.5, v/v) Authors: Mascher, H.J. et al. Source: J. Chromatogr. 506 (1990) 417–421
Details
Legend: Fig. 2. Chromatograms obtained after injection of 20µl deproteinized plasma,
(a) pre-dose plasma, (b) plasma spiked with 0.42µg/ml amoxicillin (tR 2.1min),
(c) plasma from a volunteer after a single